Evaluation of Immunogenicity of Outer Membrane Proteins of Pasteurella Multocida Serotype B:2,5 in Cattle
نویسنده
چکیده
The studies involved P. multocida of serotype B:2,5 isolated from animal affected with haemorrhagic septicaemia. Outer membrane proteins (OMPs) and iron-regulated outer membrane proteins (IROMPs) were prepared by extraction with 1% sarcosyl from the strain cultured on BHI medium and on a medium supplemented with 2,2’-dipyridyl. The antigen complexes were used to immunize calves. Each vaccine dose comprised 400 μg of OMPs suspension and 10% addition of aluminium hydroxide gel as adjuvant. The animals were injected twice at a 14-day interval. The blood was collected on the day of the first vaccination and then weekly for 6 weeks. The immunization of calves with the complexes of OMPs and IROMPs antigens caused occurrence of the specific antibodies detected with the immunoblotting and ELISA.
منابع مشابه
Genotyping of Pasteurella multocida ovine and bovine isolates from Iran based on PCR-RFLP of ompH gene
Pasteurella multocida (P. multocida), A Gram-negative facultative anaerobic bacterium, is a causative animal pathogen in porcine atrophic rhinitis and avian fowl cholera. The outer membrane of Gram-negative bacteria contains of many different protein in very high copy numbers. One of the major outer membrane, the H proteins have functional as high immunogenicity and antigenicity. In this study ...
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